formaldehyde fa solution Search Results


formaldehyde (fa) solution  (Fisher Scientific)
90
Fisher Scientific formaldehyde (fa) solution
DPCs are modified by SUMO and ubiquitin (A) <t>Formaldehyde</t> (FA) treatment promotes ubiquitylation and SUMOylation on DPCs. HeLa cells were treated with increasing concentrations of FA for 10 min at 37°C. Total DPCs were isolated by RADAR and visualized by Flamingo protein gel staining. DPCs were analyzed by western blot for the indicated post-translational modifications (PTMs). Double-stranded DNA (dsDNA) was used as a loading control to show that DPCs were isolated from the same amount of genomic DNA. (B) FA treatment causes SUMO foci formation. RPE-1 cells were treated with 1 mM FA for 10 min at 37°C. EdU was added 20 min before FA treatment in order to label dividing cells. After treatment, cells were pre-extracted, fixed, and immunostained with the indicated antibodies. Bottom panels indicate quantifications of the number of foci per nucleus. Foci were counted with ImageJ (200 nuclei), and statistical significance was calculated using an unpaired t test. Pink lines represent mean of distribution. UT, untreated; Ub, ubiquitin. (C) DPC-induced SUMO-1 foci partially co-localize with γH2AX. RPE-1 cells were treated with 1 mM FA for 10 min at 37°C. After treatment, cells were pre-extracted, fixed, and immunostained with the indicated antibodies. Right-hand side plot shows the Pearson’s coefficient for SUMO-1 and γH2Ax colocalization. The pink line represents the mean of distribution. See also <xref ref-type=Figure S1 . " width="250" height="auto" />
Formaldehyde (Fa) Solution, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/formaldehyde+fa+solution/formaldehyde++fa++solution/pmc08674535-57-0-4
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formaldehyde (fa) solution - by Bioz Stars, 2026-11
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methanol-free fa (formaldehyde) stock solutions  (TAAB Laboratories)
90
TAAB Laboratories methanol-free fa (formaldehyde) stock solutions
DPCs are modified by SUMO and ubiquitin (A) <t>Formaldehyde</t> (FA) treatment promotes ubiquitylation and SUMOylation on DPCs. HeLa cells were treated with increasing concentrations of FA for 10 min at 37°C. Total DPCs were isolated by RADAR and visualized by Flamingo protein gel staining. DPCs were analyzed by western blot for the indicated post-translational modifications (PTMs). Double-stranded DNA (dsDNA) was used as a loading control to show that DPCs were isolated from the same amount of genomic DNA. (B) FA treatment causes SUMO foci formation. RPE-1 cells were treated with 1 mM FA for 10 min at 37°C. EdU was added 20 min before FA treatment in order to label dividing cells. After treatment, cells were pre-extracted, fixed, and immunostained with the indicated antibodies. Bottom panels indicate quantifications of the number of foci per nucleus. Foci were counted with ImageJ (200 nuclei), and statistical significance was calculated using an unpaired t test. Pink lines represent mean of distribution. UT, untreated; Ub, ubiquitin. (C) DPC-induced SUMO-1 foci partially co-localize with γH2AX. RPE-1 cells were treated with 1 mM FA for 10 min at 37°C. After treatment, cells were pre-extracted, fixed, and immunostained with the indicated antibodies. Right-hand side plot shows the Pearson’s coefficient for SUMO-1 and γH2Ax colocalization. The pink line represents the mean of distribution. See also <xref ref-type=Figure S1 . " width="250" height="auto" />
Methanol Free Fa (Formaldehyde) Stock Solutions, supplied by TAAB Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/formaldehyde+fa+solution/methanol+free+fa++formaldehyde++stock+solutions/10__1042_slash_bj20090428-40-7-18
Average 90 stars, based on 1 article reviews
methanol-free fa (formaldehyde) stock solutions - by Bioz Stars, 2026-11
90/100 stars
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formaldehyde (fa) solution (ar)  (Xilong Chemical Co)
90
Xilong Chemical Co formaldehyde (fa) solution (ar)
DPCs are modified by SUMO and ubiquitin (A) <t>Formaldehyde</t> (FA) treatment promotes ubiquitylation and SUMOylation on DPCs. HeLa cells were treated with increasing concentrations of FA for 10 min at 37°C. Total DPCs were isolated by RADAR and visualized by Flamingo protein gel staining. DPCs were analyzed by western blot for the indicated post-translational modifications (PTMs). Double-stranded DNA (dsDNA) was used as a loading control to show that DPCs were isolated from the same amount of genomic DNA. (B) FA treatment causes SUMO foci formation. RPE-1 cells were treated with 1 mM FA for 10 min at 37°C. EdU was added 20 min before FA treatment in order to label dividing cells. After treatment, cells were pre-extracted, fixed, and immunostained with the indicated antibodies. Bottom panels indicate quantifications of the number of foci per nucleus. Foci were counted with ImageJ (200 nuclei), and statistical significance was calculated using an unpaired t test. Pink lines represent mean of distribution. UT, untreated; Ub, ubiquitin. (C) DPC-induced SUMO-1 foci partially co-localize with γH2AX. RPE-1 cells were treated with 1 mM FA for 10 min at 37°C. After treatment, cells were pre-extracted, fixed, and immunostained with the indicated antibodies. Right-hand side plot shows the Pearson’s coefficient for SUMO-1 and γH2Ax colocalization. The pink line represents the mean of distribution. See also <xref ref-type=Figure S1 . " width="250" height="auto" />
Formaldehyde (Fa) Solution (Ar), supplied by Xilong Chemical Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/formaldehyde+fa+solution/formaldehyde++fa++solution++ar+/pm22342837-59-0-7
Average 90 stars, based on 1 article reviews
formaldehyde (fa) solution (ar) - by Bioz Stars, 2026-11
90/100 stars
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DPCs are modified by SUMO and ubiquitin (A) Formaldehyde (FA) treatment promotes ubiquitylation and SUMOylation on DPCs. HeLa cells were treated with increasing concentrations of FA for 10 min at 37°C. Total DPCs were isolated by RADAR and visualized by Flamingo protein gel staining. DPCs were analyzed by western blot for the indicated post-translational modifications (PTMs). Double-stranded DNA (dsDNA) was used as a loading control to show that DPCs were isolated from the same amount of genomic DNA. (B) FA treatment causes SUMO foci formation. RPE-1 cells were treated with 1 mM FA for 10 min at 37°C. EdU was added 20 min before FA treatment in order to label dividing cells. After treatment, cells were pre-extracted, fixed, and immunostained with the indicated antibodies. Bottom panels indicate quantifications of the number of foci per nucleus. Foci were counted with ImageJ (200 nuclei), and statistical significance was calculated using an unpaired t test. Pink lines represent mean of distribution. UT, untreated; Ub, ubiquitin. (C) DPC-induced SUMO-1 foci partially co-localize with γH2AX. RPE-1 cells were treated with 1 mM FA for 10 min at 37°C. After treatment, cells were pre-extracted, fixed, and immunostained with the indicated antibodies. Right-hand side plot shows the Pearson’s coefficient for SUMO-1 and γH2Ax colocalization. The pink line represents the mean of distribution. See also <xref ref-type=Figure S1 . " width="100%" height="100%">

Journal: Cell Reports

Article Title: The protease SPRTN and SUMOylation coordinate DNA-protein crosslink repair to prevent genome instability

doi: 10.1016/j.celrep.2021.110080

Figure Lengend Snippet: DPCs are modified by SUMO and ubiquitin (A) Formaldehyde (FA) treatment promotes ubiquitylation and SUMOylation on DPCs. HeLa cells were treated with increasing concentrations of FA for 10 min at 37°C. Total DPCs were isolated by RADAR and visualized by Flamingo protein gel staining. DPCs were analyzed by western blot for the indicated post-translational modifications (PTMs). Double-stranded DNA (dsDNA) was used as a loading control to show that DPCs were isolated from the same amount of genomic DNA. (B) FA treatment causes SUMO foci formation. RPE-1 cells were treated with 1 mM FA for 10 min at 37°C. EdU was added 20 min before FA treatment in order to label dividing cells. After treatment, cells were pre-extracted, fixed, and immunostained with the indicated antibodies. Bottom panels indicate quantifications of the number of foci per nucleus. Foci were counted with ImageJ (200 nuclei), and statistical significance was calculated using an unpaired t test. Pink lines represent mean of distribution. UT, untreated; Ub, ubiquitin. (C) DPC-induced SUMO-1 foci partially co-localize with γH2AX. RPE-1 cells were treated with 1 mM FA for 10 min at 37°C. After treatment, cells were pre-extracted, fixed, and immunostained with the indicated antibodies. Right-hand side plot shows the Pearson’s coefficient for SUMO-1 and γH2Ax colocalization. The pink line represents the mean of distribution. See also Figure S1 .

Article Snippet: Formaldehyde (FA) solution , Fisher Scientific , Cat#F/1501/PB08.

Techniques: Modification, Ubiquitin Proteomics, Isolation, Staining, Western Blot, Control

Journal: Cell Reports

Article Title: The protease SPRTN and SUMOylation coordinate DNA-protein crosslink repair to prevent genome instability

doi: 10.1016/j.celrep.2021.110080

Figure Lengend Snippet:

Article Snippet: Formaldehyde (FA) solution , Fisher Scientific , Cat#F/1501/PB08.

Techniques: Virus, Subcloning, Recombinant, Staining, Picogreen Assay, Proliferation Assay, Flow Cytometry, DNA Extraction, Sequencing, Luciferase, Software, Transfection, Modification, Magnetic Beads, Membrane